منابع مشابه
Radioimmunoassay of human pepsinogen A and pepsinogen C.
We describe the development of radioimmunoassays to measure both human pepsinogen A and pepsinogen C concentrations in serum. The antibodies were raised in goats by immunization with purified pepsinogen A or C. The affinity constants of the respective antibodies were 20.10(10) l/mol and 7.10(10) l/mol. Pepsinogens A and C were labeled with Na 125I by the chloramine T method. The binding between...
متن کاملDevelopment of a Radioimmunoassay for Bovine Pepsinogen A
Gastric aspartic proteases (AP) are among proteolytic enzymes that are widely distributed in vertebrates. They are responsible for the digestion of dietary proteins and are synthesized as inactive precursors (prochymosin, pepsinogen and progastricsin), commonly known as zymogens. One part of their secretion by mucous neck and chief cells in the gastric mucosa (2, 22, 23) reaches the blood strea...
متن کاملRadioimmunoassay of Mammalian Type C Viral Proteins
A radioimmunoassay specific for a murine leukemia virus structural protein, the gs antigen, detects an antigenic reactivity in normal murine cells in culture and natural tissues. The assay was shown to measure an antigen that is highly related to the virion protein as shown by absorption tests, immunoadsorbent chromatography, and by analysis of linearized dose-response curves. These findings co...
متن کاملPepsinogen C proteolytic processing of surfactant protein B.
Surfactant protein B (SP-B) is essential to the function of pulmonary surfactant and to lamellar body genesis in alveolar epithelial type 2 cells. The bioactive, mature SP-B is derived from multistep post-translational proteolysis of a larger proprotein. The identity of the proteases involved in carboxyl-terminal cleavage of proSP-B remains uncertain. This cleavage event distinguishes SP-B prod...
متن کاملRadioimmunoassay for mammalian type C viral reverse transcriptase.
Radioimmunological techniques were applied to the analysis of reverse transcriptase of mammalian type C RNA viruses. The polymerase of Rauscher mouse leukemia virus was purified by ion exchange and sequential affinity chromatography. Radioimmunoassays that utilized the viral enzyme as a probe detected as little as 1 ng of purified polymerase. No cross-reactivity could be demonstrated between th...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1990
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/36.9.1690a